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KMID : 1177720190130010011
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Journal of Alternatives to Animal Experiments
2019 Volume.13 No. 1 p.11 ~ p.16
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The Stability Study of Peptide for the Spectrophotometric Direct Peptide Reactivity Assay (Spectro-DPRA) according to Storage Conditions
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Park Kyo-Hyun
Seo Jung-Ah
Kim Bae-Hwan
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Abstract
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The Spectrophotometric direct peptide reactivity assay (Spectro-DPRA) is now in process for the development and validation of evaluation on skin-sensitizing chemicals. The cysteine peptides (Ac-RFAACAACOOH) and lysine peptides (Ac-RFAAKAA-COOH) is used for Spectro-DPRA. The objective of this study is to confirm the stability of cysteine and lysine peptides which are used for Spectro-DPRA through the storage conditions. We analyzed the stability of peptide using HPLC according to the conditions, such as storage temperature and storage period. The peptide powders of cysteine and lysine were stable on storage -80℃, for 6 and 12 month. And 10 mM stock peptides of cysteine and lysine were also stable on storage -70℃, for 1, 3 and 6 month. But 10 mM stock peptides of cysteine were denatured and not stable on storage -20℃, for 1 month. Taken together, peptides powder for Spectro-DPRA should be stored under -80℃, less than 12 month. And, 10 mM stock peptides should be also stored under -70℃, less than 6 month.
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KEYWORD
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Spectro-DPRA, cysteine, lysine, peptides, stability
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